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Antibodies

RXFP2 Antibody

Product Sizes
50 ug
£186.00
CSB-PA819908LA01HU-50UG
100 ug
£334.00
CSB-PA819908LA01HU-100UG
About this Product
SKU:
CSB-PA819908LA01HU
Additional Names:
RXFP2 antibody; GPR106 antibody; GREAT antibody; LGR8Relaxin receptor 2 antibody; G-protein coupled receptor 106 antibody; G-protein coupled receptor affecting testicular descent antibody; Leucine-rich repeat-containing G-protein coupled receptor 8 antibody; Relaxin family peptide receptor 2 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Receptor for relaxin. The activity of this receptor is mediated by G proteins leading to stimulation of adenylate cyclase and an increase of cAMP. May also be a receptor for Leydig insulin-like peptide (INSL3).
Host:
Rabbit
Immunogen:
Recombinant Human Relaxin receptor 2 protein (33-158AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA819908LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA819908LA01HU diluted at 1:290 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA819908LA01HU diluted at 1:290 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: A549 whole cell lysate All lanes: RXFP2 antibody at 4.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 87, 84 kDa Observed band size: 87 kDa