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Antibodies

KTN1 Antibody

Product Sizes
50 ug
£186.00
CSB-PA773030LA01HU-50UG
100 ug
£334.00
CSB-PA773030LA01HU-100UG
About this Product
SKU:
CSB-PA773030LA01HU
Additional Names:
CG-1 antigen antibody; CG1 antibody; CG1 antigen antibody; KIAA0004 antibody; Kinectin antibody; Kinesin receptor antibody; Ktn1 antibody; KTN1_HUMAN antibody; MU-RMS-40.19 antibody
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Receptor for kinesin thus involved in kinesin-driven vesicle motility. Accumulates in integrin-based adhesion complexes (IAC) upon integrin aggregation by fibronectin.
Host:
Rabbit
Immunogen:
Recombinant Human Kinectin protein (978-1116AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
IHC image of CSB-PA773030LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA773030LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: KTN1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 157, 150, 151, 153 kDa Observed band size: 157 kDa
Immunoprecipitating KTN1 in A549 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA773030LA01HU in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA773030LA01HU (6ug) + A549 whole cell lysate (1mg) Lane 3: A549 whole cell lysate (20ug)