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Antibodies

ACOT1 Antibody

Product Sizes
50 ug
£186.00
CSB-PA768206LA01HU-50UG
100 ug
£334.00
CSB-PA768206LA01HU-100UG
About this Product
SKU:
CSB-PA768206LA01HU
Additional Names:
ACOT1 antibody; CTE1Acyl-coenzyme A thioesterase 1 antibody; Acyl-CoA thioesterase 1 antibody; EC 3.1.2.- antibody; CTE-I antibody; CTE-Ib antibody; Inducible cytosolic acyl-coenzyme A thioester hydrolase antibody; Long chain acyl-CoA thioester hydrolase antibody; Long chain acyl-CoA hydrolase antibody; Palmitoyl-coenzyme A thioesterase antibody; EC 3.1.2.2 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16 (By similarity).
Host:
Rabbit
Immunogen:
Recombinant Human Acyl-coenzyme A thioesterase 1 protein (258-350AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA768206LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA768206LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.