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Antibodies

GOLGB1 Antibody

Product Sizes
50 ul
£186.00
CSB-PA622788LA01HU-50UL
100 ul
£334.00
CSB-PA622788LA01HU-100UL
About this Product
SKU:
CSB-PA622788LA01HU
Additional Names:
372 kDa Golgi complex associated protein antibody; 372 kDa Golgi complex-associated protein antibody; GCP antibody; GCP372 antibody; Giantin antibody; GOGB1_HUMAN antibody; GOLGB1 antibody; Golgi autoantigen golgin subfamily a member 1 antibody; Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1 antibody; Golgi autoantigen, golgin subfamily b, macrogolgin 1 antibody; Golgi complex associated protein 372kDa antibody; Golgi complex-associated protein 372kDa antibody; Golgin B1 antibody; Golgin B1, golgi integral membrane protein antibody; Golgin subfamily B member 1 antibody; GOLIM1 antibody; Macrogolgin antibody; MGC33154 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
May participate in forming intercisternal cross-bridges of the Golgi complex.
Host:
Rabbit
Immunogen:
Recombinant Human Golgin subfamily B member 1 protein (1764-1869AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA622788LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA622788LA01HU diluted at 1:300 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.