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Antibodies

RAB3GAP1 Antibody

Product Sizes
50 ug
£186.00
CSB-PA621861LA01HU-50UG
100 ug
£334.00
CSB-PA621861LA01HU-100UG
About this Product
SKU:
CSB-PA621861LA01HU
Additional Names:
DKFZp434A012 antibody; KIAA0066 antibody; P130 antibody; Rab3 GAP antibody; Rab3 GAP p130 antibody; RAB3 GTPase activating protein 130 kDa subunit antibody; Rab3 GTPase activating protein catalytic subunit antibody; RAB3 GTPase activating protein subunit 1 (catalytic) antibody; RAB3 GTPase-activating protein 130 kDa subunit antibody; Rab3 GTPase-activating protein catalytic subunit antibody; Rab3-GAP antibody; Rab3-GAP p130 antibody; RAB3GAP antibody; rab3gap1 antibody; RAB3GAP130 antibody; RB3GP_HUMAN antibody; WARBM1 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Probable catalytic subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters.
Host:
Rabbit
Immunogen:
Recombinant Human Rab3 GTPase-activating protein catalytic subunit protein (527-653AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA621861LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA621861LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA621861LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.
Western Blot Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Hela whole cell lysate All lanes: RAB3GAP1 antibody at 7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 111, 112, 106 kDa Observed band size: 111 kDa