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Antibodies

PDE9A Antibody

Product Sizes
50 ug
£186.00
CSB-PA527985LA01HU-50UG
100 ug
£334.00
CSB-PA527985LA01HU-100UG
About this Product
SKU:
CSB-PA527985LA01HU
Additional Names:
5''-cyclic phosphodiesterase 9A antibody; cGMP specific 3' 5' cyclic phosphodiesterase type 9 antibody; FLJ90181 antibody; High affinity cGMP-specific 3'' antibody; High-affinity cGMP-specific 3'5'-cyclic phosphodiesterase 9A antibody; HSPDE9A2 antibody; OTTHUMP00000109399 antibody; PDE 9A antibody; Pde9a antibody; PDE9A_HUMAN antibody; Phosphodiesterase 9A antibody; phosphodiesterase PDE9A21 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP (PubMed:9624146, PubMed:18757755, PubMed:21483814). Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed:25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (By similarity).
Host:
Rabbit
Immunogen:
Recombinant Human High affinity cGMP-specific 3\\\',5\\\'-cyclic phosphodiesterase 9A protein (426-533AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of A549 cells with CSB-PA527985LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA527985LA01HU diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: A549 whole cell lysate All lanes: PDE9A antibody at 3.9ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 62, 55, 63, 58, 46, 59, 51, 45, 54, 65, 66, 57 kDa Observed band size: 69 kDa