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Antibodies

ATP2B4 Antibody

Product Sizes
50 ug
£186.00
CSB-PA23389A0RB-50UG
100 ug
£334.00
CSB-PA23389A0RB-100UG
About this Product
SKU:
CSB-PA23389A0RB
Additional Names:
AT2B4_HUMAN antibody; ATP2B2 antibody; Atp2b4 antibody; ATPase Ca++ transporting plasma membrane 4 antibody; Cation transporting ATPase antibody; DKFZp686G08106 antibody; DKFZp686M088 antibody; Matrix remodelling associated 1 antibody; Matrix remodelling associated protein 1 antibody; Matrix-remodeling-associated protein 1 antibody; MXRA 1 antibody; MXRA1 antibody; OTTHUMP00000034088 antibody; OTTHUMP00000034089 antibody; Plasma membrane calcium ATPase 4 antibody; Plasma membrane calcium ATPase isoform 4 antibody; Plasma membrane calcium pump isoform 4 antibody; Plasma membrane calcium transporting ATPase 4 antibody; Plasma membrane calcium-transporting ATPase 4 antibody; PMCA 4 antibody; PMCA4 antibody; PMCA4b antibody; PMCA4x antibody; Sarcolemmal calcium pump antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Calcium/calmodulin-regulated and magnesium-dependent enzyme that catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell (PubMed:8530416). By regulating sperm cell calcium homeostasis, may play a role in sperm motility (By similarity).
Host:
Rabbit
Immunogen:
Recombinant Human Plasma membrane calcium-transporting ATPase 4 protein (281-345AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA23389A0Rb at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA23389A0Rb diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA23389A0Rb diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.