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Antibodies

MSLN Antibody

Product Sizes
50 ug
£186.00
CSB-PA10159A0RB-50UG
100 ug
£334.00
CSB-PA10159A0RB-100UG
About this Product
SKU:
CSB-PA10159A0RB
Additional Names:
CAK 1 antibody; CAK1 antibody; CAK1 antigen antibody; cleaved form antibody; Megakaryocyte potentiating factor antibody; Mesothelin antibody; Mesothelin isoform 1 precursor antibody; MPF antibody; Msln antibody; MSLN_HUMAN antibody; Pre pro megakaryocyte potentiating factor antibody; Pre-pro-megakaryocyte-potentiating factor antibody; SMR antibody; SMRP antibody; Soluble MPF mesothelin related protein antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Extra Details:
Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Host:
Rabbit
Immunogen:
Recombinant Human Mesothelin protein (37-598AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
IHC image of CSB-PA10159A0Rb diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-PA10159A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA10159A0Rb diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating MSLN in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA10159A0Rb in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA10159A0Rb (8ug) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (10ug)