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Antibodies

MT3 Antibody

Product Sizes
50 ug
£186.00
CSB-PA04639A0RB-50UG
100 ug
£334.00
CSB-PA04639A0RB-100UG
About this Product
SKU:
CSB-PA04639A0RB
Additional Names:
GIF antibody; GIFB antibody; GRIF antibody; Growth inhibitory factor antibody; Metallothionein 3 (growth inhibitory factor (neurotrophic)) antibody; Metallothionein 3 antibody; Metallothionein III antibody; Metallothionein-3 antibody; Metallothionein-III antibody; Mt 3 antibody; MT III antibody; MT-3 antibody; MT-III antibody; MT3 antibody; MT3_HUMAN antibody; ZnMT3 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.
Host:
Rabbit
Immunogen:
Recombinant Human Metallothionein-3 protein (1-68AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA04639A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA04639A0Rb diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.