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Antibodies

PSAP Antibody

Product Sizes
50 ug
£186.00
CSB-PA018836DA01HU-50UG
100 ug
£334.00
CSB-PA018836DA01HU-100UG
About this Product
SKU:
CSB-PA018836DA01HU
Additional Names:
A1 activator antibody; Cerebroside sulfate activator antibody; Co-beta-glucosidase antibody; Component C antibody; CSAct antibody; Dispersin antibody; GLBA antibody; Glucosylceramidase activator antibody; Proactivator polypeptide antibody; Proactivator polypeptide precursor antibody; Prosaposin (sphingolipid activator protein 1) antibody; prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) antibody; Prosaposin antibody; Protein A antibody; Protein C antibody; PSAP antibody; SAP 1 antibody; SAP 2 antibody; SAP-1 antibody; SAP-2 antibody; SAP_HUMAN antibody; SAP1 antibody; Saposin A antibody; Saposin B antibody; Saposin B Val antibody; Saposin C antibody; Saposin D antibody; Saposin-D antibody; Saposins antibody; Sgp1 antibody; Sphingolipid activator protein 1 antibody; Sphingolipid activator protein 2 antibody; Sulfated glycoprotein 1 antibody; Sulfatide/GM1 activator antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Extra Details:
The lysosomal degradation of sphingolipids takes place by the sequential action of specific hydrolases. Some of these enzymes require specific low-molecular mass, non-enzymic proteins: the sphingolipids activator proteins (coproteins).
Host:
Rabbit
Immunogen:
Recombinant Human Prosaposin protein (311-391AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Western Blot Positive WB detected in: Mouse heart tissue All lanes: PSAP antibody at 3.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa
Immunofluorescence staining of MCF-7 cells with CSB-PA018836DA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA018836DA01HU diluted at 1:500 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA018836DA01HU diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.