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Antibodies

PHB Antibody

Product Sizes
50 ug
£186.00
CSB-PA017885LA01HU-50UG
100 ug
£334.00
CSB-PA017885LA01HU-100UG
About this Product
SKU:
CSB-PA017885LA01HU
Additional Names:
Epididymis luminal protein 215 antibody; Epididymis secretory sperm binding protein Li 54e antibody; HEL 215 antibody; HEL S 54e antibody; PHB antibody; PHB_HUMAN antibody; PHB1 antibody; Prohibitin antibody
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Extra Details:
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Host:
Rabbit
Immunogen:
Recombinant Human Prohibitin protein (1-272AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
IHC image of CSB-PA017885LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating PHB in HEK293 whole cell lysate Lane 1: Rabbit control IgG (1ug) instead of CSB-PA017885LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA017885LA01HU (8ug) + HEK293 whole cell lysate (500ug) Lane 3: HEK293 whole cell lysate (10ug)
Western Blot Positive WB detected in: Raji whole cell lysate All lanes: PHB antibody at 4.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 18 kDa Observed band size: 30 kDa