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Antibodies

PAM Antibody

Product Sizes
50 ug
£186.00
CSB-PA017417LA01HU-50UG
100 ug
£334.00
CSB-PA017417LA01HU-100UG
About this Product
SKU:
CSB-PA017417LA01HU
Additional Names:
AMD_HUMAN antibody; PAL antibody; PAM antibody; Pancreatic peptidylglycine alpha amidating monooxygenase antibody; Peptidyl alpha amidating enzyme antibody; Peptidyl alpha hydroxyglycine alpha amidating lyase antibody; Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody; Peptidylamidoglycolate lyase antibody; Peptidylglycine 2 hydroxylase antibody; Peptidylglycine alpha amidating monooxygenase antibody; Peptidylglycine alpha hydroxylating monooxygenase antibody; PHM antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
Host:
Rabbit
Immunogen:
Recombinant Human Peptidyl-glycine alpha-amidating monooxygenase protein (338-497AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA017417LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA017417LA01HU diluted at 1:200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA017417LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: PAM antibody at 5.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 109, 97, 101, 99, 107 kDa Observed band size: 109 kDa