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Antibodies

LPAR6 Antibody

Product Sizes
50 ul
£186.00
CSB-PA017336OA01HU-50UL
100 ul
£334.00
CSB-PA017336OA01HU-100UL
About this Product
SKU:
CSB-PA017336OA01HU
Additional Names:
ARWH1 antibody; G protein coupled purinergic receptor P2Y5 antibody; LAH 3 antibody; LAH3 antibody; LPA receptor 6 antibody; LPA-6 antibody; LPAR 6 antibody; Lpar6 antibody; LPAR6_HUMAN antibody; Lysophosphatidic acid receptor 6 antibody; MGC 120358 antibody; MGC120358 antibody; Oleoyl L alpha lysophosphatidic acid receptor antibody; Oleoyl-L-alpha-lysophosphatidic acid receptor antibody; P 2 RY 5 antibody; p2ry5 antibody; P2Y purinoceptor 5 antibody; P2Y5 antibody; Purinergic receptor 5 antibody; purinergic receptor P2Y, G protein coupled, 5 antibody; purinergic receptor p2y5 antibody; RB intron encoded G protein coupled receptor antibody; RB intron encoded G-protein coupled receptor antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Binds to oleoyl-L-alpha-lysophosphatidic acid (LPA). Intracellular cAMP is involved in the receptor activation. Important for the maintenance of hair growth and texture.
Host:
Rabbit
Immunogen:
Peptide sequence from Human Lysophosphatidic acid receptor 6 protein (292-313AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA017336OA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA017336OA01HU diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate All lanes: LPAR6 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa