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Antibodies

OCLN Antibody

Product Sizes
50 ug
£186.00
CSB-PA016263LA01HU-50UG
100 ug
£334.00
CSB-PA016263LA01HU-100UG
About this Product
SKU:
CSB-PA016263LA01HU
Additional Names:
BLCPMG antibody; FLJ08163 antibody; FLJ18079 antibody; FLJ77961 antibody; FLJ94056 antibody; MGC34277 antibody; Occludin antibody; Ocln antibody; OCLN_HUMAN antibody; Phosphatase 1 regulatory subunit 115 antibody; PPP1R115 antibody; PTORCH1 antibody; Tight junction protein occludin antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Host:
Rabbit
Immunogen:
Recombinant Human Occludin protein (285-430AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of MCF-7 cells with CSB-PA016263LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA016263LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: OCLN antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 53, 55, 32, 24, 9 kDa Observed band size: 60 kDa