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Antibodies

MDH1 Antibody

Product Sizes
50 ul
£186.00
CSB-PA013621ESR1HU-50UL
100 ul
£334.00
CSB-PA013621ESR1HU-100UL
About this Product
SKU:
CSB-PA013621ESR1HU
Additional Names:
cytoplasmic antibody; Cytosolic malate dehydrogenase antibody; Diiodophenylpyruvate reductase antibody; Malate dehydrogenase 1, NAD (soluble) antibody; Malate dehydrogenase antibody; Malate dehydrogenase cytoplasmic antibody; MDH s antibody; mdh1 antibody; MDHA antibody; MDHC_HUMAN antibody; MDHs antibody; MGC:1375 antibody; MOR2 antibody; Soluble malate dehydrogenase antibody
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
Clonality:
Polyclonal
Extra Details:
MDH1(Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.
Host:
Rabbit
Immunogen:
Recombinant Human Malate dehydrogenase, cytoplasmic protein (1-334AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA013621ESR1HU at dilution of 1:100
Western blot All lanes: MDH1 antibody at 4ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37, 28, 39 kDa Observed band size: 37 kDa
Immunoprecipitating MDH1 in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA013621ESR1HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA013621ESR1HU (4ug) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (20ug)