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Antibodies

MCM2 Antibody

Product Sizes
50 ug
£186.00
CSB-PA013590LA01HU-50UG
100 ug
£334.00
CSB-PA013590LA01HU-100UG
About this Product
SKU:
CSB-PA013590LA01HU
Additional Names:
BM28 antibody; CCNL 1 antibody; CCNL1 antibody; CDC like 1 antibody; cdc19 antibody; CDCL 1 antibody; CDCL1 antibody; Cell devision cycle like 1 antibody; Cyclin like 1 antibody; D3S3194 antibody; DNA replication licensing factor MCM2 antibody; KIAA0030 antibody; MCM 2 antibody; MCM2 antibody; MCM2 minichromosome maintenance deficient 2 mitotin antibody; MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody; MCM2 minichromosome maintenance deficient 2; mitotin antibody; MCM2_HUMAN antibody; MGC10606 antibody; Minichromosome maintenance complex component 2 antibody; Minichromosome maintenance deficient 2 (mitotin) antibody; Minichromosome maintenance deficient 2 mitotin antibody; Minichromosome maintenance protein 2 antibody; Minichromosome maintenance protein 2 homolog antibody; Mitotin antibody; Nuclear protein BM28 antibody; OTTHUMP00000216047 antibody; OTTHUMP00000216050 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Extra Details:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Host:
Rabbit
Immunogen:
Recombinant Human DNA replication licensing factor MCM2 protein (2-904AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of MCF-7 cells with CSB-PA013590LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA013590LA01HU diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate All lanes: MCM2 antibody at 4.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 102 kDa Observed band size: 102 kDa