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Antibodies

MAS1 Antibody

Product Sizes
50 ug
£186.00
CSB-PA013505LA01HU-50UG
100 ug
£334.00
CSB-PA013505LA01HU-100UG
About this Product
SKU:
CSB-PA013505LA01HU
Additional Names:
MAS antibody; MAS proto oncogene antibody; Mas-related G protein-coupled receptor A antibody; MAS_HUMAN antibody; Mas1 antibody; MAS1 oncogene antibody; MAS1 proto-oncogene; G protein-coupled receptor antibody; MGRA antibody; Proto-oncogene Mas antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Extra Details:
Receptor for angiotensin 1-7 (By similarity). Acts specifically as a functional antagonist of AGTR1 (angiotensin-2 type 1 receptor), although it up-regulates AGTR1 receptor levels. Positive regulation of AGTR1 levels occurs through activation of the G-proteins GNA11 and GNAQ, and stimulation of the protein kinase C signaling cascade. The antagonist effect on AGTR1 function is probably due to AGTR1 being physically altered by MAS1.
Host:
Rabbit
Immunogen:
Recombinant Human Proto-oncogene Mas protein (285-325AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA013505LA01HU at 1:200, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA013505LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: Mouse Brain tissue All lanes: MAS1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa