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Antibodies

MAGEA3 Antibody

Product Sizes
50 ug
£186.00
CSB-PA013329LA01HU-50UG
100 ug
£334.00
CSB-PA013329LA01HU-100UG
About this Product
SKU:
CSB-PA013329LA01HU
Additional Names:
Antigen MZ2 D antibody; Antigen MZ2-D antibody; Cancer/testis antigen 1.3 antibody; Cancer/testis antigen family 1 member 3 antibody; CT1.3 antibody; HIP8 antibody; HYPD antibody; MAGA3_HUMAN antibody; MAGE 3 antigen antibody; MAGE family member A3 antibody; MAGE-3 antigen antibody; MAGE3 antibody; MAGEA3 antibody; MAGEA6 antibody; Melanoma antigen family A 3 antibody; Melanoma associated antigen 3 antibody; Melanoma-associated antigen 3 antibody; MGC1461 antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
Host:
Rabbit
Immunogen:
Recombinant Human Melanoma-associated antigen 3 protein (2-104AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
IHC image of CSB-PA013329LA01HU diluted at 1:200 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA013329LA01HU diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: A549 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate All lanes: MAGEA3 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa