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Antibodies

LMAN1 Antibody

Product Sizes
50 ug
£186.00
CSB-PA012991LA01HU-50UG
100 ug
£334.00
CSB-PA012991LA01HU-100UG
About this Product
SKU:
CSB-PA012991LA01HU
Additional Names:
Endoplasmic reticulum golgi intermediate compartment protein 53 antibody; ER-Golgi intermediate compartment 53 kDa protein antibody; ERGIC-53 antibody; ERGIC53 antibody; ERGIC53 like protein antibody; F5F8D antibody; FMFD1 antibody; Gp58 antibody; Intracellular mannose specific lectin antibody; Intracellular mannose-specific lectin MR60 antibody; Lectin mannose binding 1 antibody; Lectin mannose-binding 1 antibody; Lman1 antibody; LMAN1 like protein antibody; LMAN1_HUMAN antibody; MCFD1 antibody; MR60 antibody; Protein ERGIC-53 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
Host:
Rabbit
Immunogen:
Recombinant Human Protein ERGIC-53 protein (154-329AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of A549 cells with CSB-PA012991LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA012991LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue All lanes: LMAN1 antibody at 5.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa