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Antibodies

LETM1 Antibody

Product Sizes
50 ug
£186.00
CSB-PA012877LA01HU-50UG
100 ug
£334.00
CSB-PA012877LA01HU-100UG
About this Product
SKU:
CSB-PA012877LA01HU
Additional Names:
LETM 1 antibody; LETM1 and EF hand domain containing protein 1; mitochondrial antibody; LETM1 and EF-hand domain-containing protein 1 antibody; Letm1 antibody; LETM1_HUMAN antibody; Leucine zipper EF hand containing transmembrane protein 1 antibody; Leucine zipper-EF-hand-containing transmembrane protein 1 antibody; Mdm38 homolog antibody; mitochondrial antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Mitochondrial proton/calcium antiporter that mediates proton-dependent calcium efflux from mitochondrion (PubMed:19797662). Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain (PubMed:18628306). Required for the maintenance of the tubular shape and cristae organization (PubMed:18628306). In contrast to SLC8B1/NCLX, does not constitute the major factor for mitochondrial calcium extrusion (PubMed:24898248).
Host:
Rabbit
Immunogen:
Recombinant Human Mitochondrial proton/calcium exchanger protein (443-543AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA012877LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA012877LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, Mouse heart tissue All lanes: LETM1 antibody at 6.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84, 30, 34 kDa Observed band size: 84 kDa