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Antibodies

HNRNPAB Antibody

Product Sizes
50 ug
£186.00
CSB-PA010604LA01HU-50UG
100 ug
£334.00
CSB-PA010604LA01HU-100UG
About this Product
SKU:
CSB-PA010604LA01HU
Additional Names:
ABBP 1 antibody; ABBP-1 antibody; ABBP1 antibody; Apobec 1 binding protein 1 antibody; APOBEC1 binding protein 1 antibody; APOBEC1-binding protein 1 antibody; Apolipoprotein B mRNA editing enzyme catalytic polypeptide 1- protein 1 antibody; FLJ40338 antibody; Heterogeneous nuclear ribonucleoprotein A/B antibody; hnRNP A/B antibody; hnRNP type A/B protein antibody; HNRNPAB antibody; HNRPAB antibody; ROAA_HUMAN antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Binds single-stranded RNA. Has a high affinity for G-rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site.
Host:
Rabbit
Immunogen:
Recombinant Human Heterogeneous nuclear ribonucleoprotein A/B protein (1-68AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA010604LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA010604LA01HU diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA010604LA01HU diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: K562 whole cell lysate All lanes: HNRNPAB antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 36, 31 kDa Observed band size: 37, 36 kDa