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Antibodies

Acetyl-HIST1H2BB (K16) Antibody

Product Sizes
50 ul
£195.00
CSB-PA010402OA16ACHU-50UL
100 ul
£356.00
CSB-PA010402OA16ACHU-100UL
About this Product
SKU:
CSB-PA010402OA16ACHU
Additional Names:
HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody
Application:
ChIP, ELISA, Immunocytochemistry, Immunofluorescence
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Host:
Rabbit
Immunogen:
Peptide sequence around site of Acetyl-Lys (16) derived from Human Histone H2B type 1-B
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8ug anti-HIST1H2BB (CSB-PA010402OA16acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the B Beta-Globin promoter.
Immunocytochemistry analysis of Hela cells using CSB-PA010402OA16acHU at dilution of 1:100
Immunofluorescent analysis of Hela cells treated with NaB using CSB-PA010402OA16acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)