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Antibodies

B Beta-hydroxybutyryl-HIST1H1D (K75) Antibody

Product Sizes
50 ul
£195.00
CSB-PA010379OA75BHBHU-50UL
100 ul
£356.00
CSB-PA010379OA75BHBHU-100UL
About this Product
SKU:
CSB-PA010379OA75BHBHU
Additional Names:
H1 histone family member 3 antibody; H1.3 antibody; H13_HUMAN antibody; H1F3 antibody; HIST1 H1D antibody; HIST1H1D antibody; Histone 1 H1d antibody; Histone cluster 1 H1d antibody; Histone H1.3 antibody; Histone H1c antibody; MGC138176 antibody
Application:
ELISA, Immunocytochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Host:
Rabbit
Immunogen:
Peptide sequence around site of B Beta-hydroxybutyryl-Lys (75) derived from Human Histone H1.3
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Western Blot Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate (all treated with 30mM sodium butyrate for 4h) All lanes: HIST1H1D antibody at 1.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
Immunocytochemistry analysis of CSB-PA010379OA75bhbHU diluted at 1:10 and staining in Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.