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Antibodies

SULT1A3 Antibody

Product Sizes
50 ug
£186.00
CSB-PA00887A0RB-50UG
100 ug
£334.00
CSB-PA00887A0RB-100UG
About this Product
SKU:
CSB-PA00887A0RB
Additional Names:
SULT1A3 antibody; STM antibody; Sulfotransferase 1A3 antibody; ST1A3 antibody; EC 2.8.2.1 antibody; Aryl sulfotransferase 1A3/1A4 antibody; Catecholamine-sulfating phenol sulfotransferase antibody; HAST3 antibody; M-PST antibody; Monoamine-sulfating phenol sulfotransferase antibody; Placental estrogen sulfotransferase antibody; Sulfotransferase 1A3/1A4 antibody; Sulfotransferase antibody; monoamine-preferring antibody; Thermolabile phenol sulfotransferase antibody; TL-PST antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Extra Details:
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.
Host:
Rabbit
Immunogen:
Recombinant Human Sulfotransferase 1A3 protein (1-295AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Western blot All lanes: SULT1A3 antibody at 20ug/ml Lane 1: A549 whole cell lysate Lane 2: Colo320 whole cell lysate Lane 3: Mouse brain tissue Lane 4: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 23 kDa Observed band size: 35 kDa
Immunofluorescence staining of HepG2 cells with CSB-PA00887A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA00887A0Rb diluted at 1:1000 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.