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Antibodies

FGF1 Antibody

Product Sizes
50 ul
£186.00
CSB-PA008615ESR1HU-50UL
100 ul
£334.00
CSB-PA008615ESR1HU-100UL
About this Product
SKU:
CSB-PA008615ESR1HU
Additional Names:
Acidic fibroblast growth factor antibody; aFGF antibody; Beta endothelial cell growth factor antibody; Beta-endothelial cell growth factor antibody; ECGF antibody; ECGF beta antibody; ECGF-beta antibody; ECGFA antibody; ECGFB antibody; Endothelial Cell Growth Factor alpha antibody; Endothelial Cell Growth Factor beta antibody; FGF 1 antibody; FGF alpha antibody; Fgf1 antibody; FGF1_HUMAN antibody; FGFA antibody; Fibroblast Growth Factor 1 Acidic antibody; Fibroblast growth factor 1 antibody; GLIO703 antibody; HBGF 1 antibody; HBGF-1 antibody; HBGF1 antibody; Heparin binding growth factor 1 antibody; Heparin binding growth factor 1 precursor antibody; Heparin-binding growth factor 1 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
Clonality:
Polyclonal
Extra Details:
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
Host:
Rabbit
Immunogen:
Recombinant Human Fibroblast growth factor 1 protein (16-155AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA008615ESR1HU at dilution of 1:100
Immunofluorescence staining of Hela cells with CSB-PA008615ESR1HU at 1:96, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA008615ESR1HU diluted at 1:289 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA008615ESR1HU diluted at 1:289 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot All lanes: Fibroblast growth factor 1 antibody at 6ug/ml Lane 1: Mouse kidney tissue Lane 2: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 18, 7 kDa Observed band size: 18 kDa