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Antibodies

EMD Antibody

Product Sizes
50 ug
£186.00
CSB-PA007633LA01HU-50UG
100 ug
£334.00
CSB-PA007633LA01HU-100UG
About this Product
SKU:
CSB-PA007633LA01HU
Additional Names:
EDMD antibody; Emd antibody; EMD_HUMAN antibody; Emerin antibody; Emery Dreifuss muscular dystrophy antibody; STA antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
Host:
Rabbit
Immunogen:
Recombinant Human Emerin protein (1-222AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescent analysis of Hela cells using CSB-PA007633LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA007633LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human bladder cancer using CSB-PA007633LA01HU at dilution of 1:100
Immunoprecipitating EMD in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA007633LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA007633LA01HU (8ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (10ug)
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Colo320 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Mouse lung tissue All lanes: EMD antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 35 kDa