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Antibodies

DHCR7 Antibody

Product Sizes
50 ug
£186.00
CSB-PA006844LA01HU-50UG
100 ug
£334.00
CSB-PA006844LA01HU-100UG
About this Product
SKU:
CSB-PA006844LA01HU
Additional Names:
DHCR7 antibody; D7SR antibody; 7-dehydrocholesterol reductase antibody; 7-DHC reductase antibody; EC 1.3.1.21 antibody; Delta7-sterol reductase antibody; Sterol Delta(7)-reductase antibody; Sterol reductase SR-2 antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
Host:
Rabbit
Immunogen:
Recombinant Human 7-dehydrocholesterol reductase protein (63-147AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
IHC image of CSB-PA006844LA01HU diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate All lanes: DHCR7 antibody at 2.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa