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Antibodies

CYP21A2 Antibody

Product Sizes
50 ug
£186.00
CSB-PA006400LA01HU-50UG
100 ug
£334.00
CSB-PA006400LA01HU-100UG
About this Product
SKU:
CSB-PA006400LA01HU
Additional Names:
CYP21 antibody; 21 OHase antibody; 21-OHase antibody; CA21H antibody; CAH1 antibody; CP21A_HUMAN antibody; CPS1 antibody; CYP21A 2 antibody; CYP21A2 antibody; CYP21B antibody; Cytochrome P-450c21 antibody; Cytochrome P450 21 antibody; Cytochrome P450 C21B antibody; Cytochrome P450 XXI antibody; Cytochrome P450; family 21; subfamily A; polypeptide 2 antibody; Cytochrome P450; subfamily XXIA (steroid 21 hydroxylase; congenital adrenal hyperplasia); polypeptide 2 antibody; Cytochrome P450-C21 antibody; Cytochrome P450-C21B antibody; P450 C21 antibody; P450 C21B antibody; P450c21B antibody; Steroid 21 hydroxylase antibody; Steroid 21 monooxygenase antibody; Steroid 21-hydroxylase antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant Human Steroid 21-hydroxylase protein (312-418AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 with CSB-PA006400LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA006400LA01HU diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Jurkat whole cell lysate, HL60 whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate All lanes: CYP21A2 antibody at 8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 53 kDa Observed band size: 56 kDa