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Antibodies

CRYAB Antibody

Product Sizes
50 ug
£186.00
CSB-PA006008HA01HU-50UG
100 ug
£334.00
CSB-PA006008HA01HU-100UG
About this Product
SKU:
CSB-PA006008HA01HU
Additional Names:
AACRYA antibody; Alpha B crystallin antibody; Alpha crystallin B chain antibody; Alpha(B)-crystallin antibody; Alpha-crystallin B chain antibody; CRYA2 antibody; Cryab antibody; CRYAB_HUMAN antibody; Crystallin alpha B antibody; Crystallin alpha polypeptide 2 antibody; CTPP2 antibody; Heat shock 20 kD like protein antibody; Heat shock protein beta 5 antibody; Heat shock protein beta-5 antibody; HspB5 antibody; Renal carcinoma antigen NY REN 27 antibody; Renal carcinoma antigen NY-REN-27 antibody; Rosenthal fiber component antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Extra Details:
May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
Host:
Rabbit
Immunogen:
Recombinant Human Alpha-crystallin B chain protein (1-175AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
IHC image of CSB-PA006008HA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Rat heart tissue, Mouse heart tissue All lanes: CRYAB antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa
Immunofluorescence staining of U251 cells with CSB-PA006008HA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA006008HA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.