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Antibodies

CRB1 Antibody

Product Sizes
50 ul
£186.00
CSB-PA005941DSR1HU-50UL
100 ul
£334.00
CSB-PA005941DSR1HU-100UL
About this Product
SKU:
CSB-PA005941DSR1HU
Additional Names:
CRB1 antibody; CRUM1_HUMAN antibody; Protein crumbs homolog 1 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
PBS
Clonality:
Polyclonal
Extra Details:
Plays a role in photoreceptor morphogenesis in the retina. May maintain cell polarization and adhesion.
Host:
Rabbit
Immunogen:
Recombinant Human Protein crumbs homolog 1 protein (20-200AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA005941DSR1HU at 1:22, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA005941DSR1HU diluted at 1:66 and staining in paraffin-embedded human eye tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.