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Antibodies

CPS1 Antibody

Product Sizes
50 ug
£186.00
CSB-PA005913LA01HU-50UG
100 ug
£334.00
CSB-PA005913LA01HU-100UG
About this Product
SKU:
CSB-PA005913LA01HU
Additional Names:
Carbamoyl phosphate synthase [ammonia] antibody; Carbamoyl phosphate synthase [ammonia] mitochondrial antibody; Carbamoyl phosphate synthase antibody; Carbamoyl phosphate synthetase 1 antibody; Carbamoyl phosphate synthetase 1 mitochondrial antibody; Carbamoyl phosphate synthetase I antibody; Carbamoyl-phosphate synthase [ammonia] antibody; Carbamoyl-phosphate synthetase I antibody; Carbamoylphosphate synthase antibody; Carbamoylphosphate synthetase 1 antibody; Carbamoylphosphate synthetase I antibody; CPS 1 antibody; Cps1 antibody; CPSase 1 antibody; CPSase I antibody; CPSASE1 antibody; CPSM_HUMAN antibody; mitochondrial antibody; MS738 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
Host:
Rabbit
Immunogen:
Recombinant Human Carbamoyl-phosphate synthase [ammonia], mitochondrial protein (1361-1500AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA005913LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA005913LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA005913LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: CPS1 antibody at 3.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 165, 117, 166 kDa Observed band size: 165 kDa
Immunoprecipitating CPS1 in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA005913LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA005913LA01HU (6ug) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (20ug)