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Antibodies

CHKA Antibody

Product Sizes
50 ug
£186.00
CSB-PA005353LA01HU-50UG
100 ug
£334.00
CSB-PA005353LA01HU-100UG
About this Product
SKU:
CSB-PA005353LA01HU
Additional Names:
CHKA antibody; CHK antibody; CKI antibody; Choline kinase alpha antibody; CK antibody; EC 2.7.1.32 antibody; CHETK-alpha antibody; Ethanolamine kinase antibody; EK antibody; EC 2.7.1.82 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline.
Host:
Rabbit
Immunogen:
Recombinant Human Choline kinase alpha protein (79-173AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA005353LA01HU at dilution of 1:100
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CHKA antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 51 kDa Observed band size: 53 kDa
Immunofluorescence staining of HepG2 cells with CSB-PA005353LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).