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Antibodies

CENPK Antibody

Product Sizes
50 ul
£186.00
CSB-PA005214ESR1HU-50UL
100 ul
£334.00
CSB-PA005214ESR1HU-100UL
About this Product
SKU:
CSB-PA005214ESR1HU
Additional Names:
CENPK antibody; ICEN37 antibody; FKSG14 antibody; Centromere protein K antibody; CENP-K antibody; Interphase centromere complex protein 37 antibody; Protein AF-5alpha antibody; p33 antibody
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
Clonality:
Polyclonal
Extra Details:
Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore.
Host:
Rabbit
Immunogen:
Recombinant Human Centromere protein K protein (1-269AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA005214ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA005214ESR1HU at dilution of 1:100
Western blot All lanes: CENPK antibody at 3.98ug/ml Lane 1: PC-3 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa
Immunoprecipitating CENPK in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA005214ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA005214ESR1HU (8ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (10ug)