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Antibodies

CCND3 Antibody

Product Sizes
50 ul
£186.00
CSB-PA004814ESR2HU-50UL
100 ul
£334.00
CSB-PA004814ESR2HU-100UL
About this Product
SKU:
CSB-PA004814ESR2HU
Additional Names:
CCND 3 antibody; Ccnd3 antibody; CCND3_HUMAN antibody; CyclinD3 antibody; D3 type cyclin antibody; G1 S specific cyclin D3 antibody; G1/S specific cyclin D3 antibody; G1/S-specific cyclin-D3 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
Clonality:
Polyclonal
Extra Details:
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Host:
Rabbit
Immunogen:
Recombinant Human G1/S-specific cyclin-D3 protein (1-292AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA004814ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA004814ESR2HU at dilution of 1:100
Immunofluorescence staining of A549 cells with CSB-PA004814ESR2HU at 1:113, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating CCND3 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA004814ESR2HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA004814ESR2HU (5ug) + Jurkat whole cell lysate (500ug) Lane 3: Jurkat whole cell lysate (20ug)
Western Blot Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate All lanes: CCND3 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa