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Antibodies

CCND2 Antibody

Product Sizes
50 ul
£186.00
CSB-PA004812ESR2HU-50UL
100 ul
£334.00
CSB-PA004812ESR2HU-100UL
About this Product
SKU:
CSB-PA004812ESR2HU
Additional Names:
CCND 2 antibody; ccnd2 antibody; CCND2_HUMAN antibody; CyclinD2 antibody; G1/S specific cyclin D2 antibody; G1/S-specific cyclin-D2 antibody; KIAK0002 antibody; MGC102758 antibody; MPPH3 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
Clonality:
Polyclonal
Extra Details:
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Host:
Rabbit
Immunogen:
Recombinant Human G1/S-specific cyclin-D2 protein (1-289AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA004812ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA004812ESR2HU at dilution of 1:100
Immunofluorescence staining of HepG2 cells with CSB-PA004812ESR2HU at 1:75, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA004812ESR2HU diluted at 1:225 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA004812ESR2HU diluted at 1:225 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.