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Antibodies

CAPN2 Antibody

Product Sizes
50 ug
£186.00
CSB-PA004496LA01HU-50UG
100 ug
£334.00
CSB-PA004496LA01HU-100UG
About this Product
SKU:
CSB-PA004496LA01HU
Additional Names:
Calcium activated neutral proteinase 2 antibody; Calcium activated neutral proteinase antibody; Calcium-activated neutral protease 2; catalytic subunit antibody; Calcium-activated neutral proteinase 2 antibody; CALP80 antibody; Calpain 2 (m/II) large subunit antibody; Calpain 2 catalytic subunit antibody; Calpain 2 large catalytic subunit antibody; Calpain 2 large subunit antibody; Calpain 2; large [catalytic] subunit antibody; Calpain large polypeptide L2 antibody; Calpain M type antibody; Calpain M-type antibody; Calpain-2 catalytic subunit antibody; Calpain-2 large subunit antibody; Calpain2 antibody; CAN2_HUMAN antibody; CANP 2 antibody; CANP L2 antibody; CANP2 antibody; CANPL 2 antibody; CANPL2 antibody; CANPml antibody; Capa2 antibody; CAPN 2 antibody; CAPN2 antibody; FLJ39928 antibody; M calpain antibody; M calpin antibody; M type antibody; M-calpain antibody; mCANP antibody; Millimolar calpain antibody; Millimolar-calpain antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Extra Details:
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Host:
Rabbit
Immunogen:
Recombinant Human Calpain-2 catalytic subunit protein (20-700AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA004496LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA004496LA01HU diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA004496LA01HU diluted at 1:300 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating CAPN2 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA004496LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA004496LA01HU (8ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (10ug)
Western Blot Positive WB detected in: 293T whole cell lysate All lanes: CAPN2 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80, 72 kDa Observed band size: 80 kDa