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Antibodies

CSTB Antibody

Product Sizes
50 ug
£186.00
CSB-PA00224A0RB-50UG
100 ug
£334.00
CSB-PA00224A0RB-100UG
About this Product
SKU:
CSB-PA00224A0RB
Additional Names:
CHROW21 antibody; CPI B antibody; CPI-B antibody; CST 6 antibody; CST6 antibody; CSTB antibody; Cystatin B (stefin B) antibody; Cystatin B antibody; Cystatin-B antibody; CYTB antibody; CYTB_HUMAN antibody; EPM1 antibody; EPM1A antibody; Liver thiol proteinase inhibitor antibody; PME antibody; Stefin-B antibody; STF B antibody; STFB antibody; ULD antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Extra Details:
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis
Host:
Rabbit
Immunogen:
Recombinant Human Cystatin-B protein (1-98AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00224A0Rb at dilution of 1:100
IHC image of CSB-PA00224A0Rb diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-PA00224A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating CSTB in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA00224A0Rb in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA00224A0Rb (6ug) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (20ug)
Western Blot Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, 293T whole cell lysate All lanes: CSTB antibody at 5.36ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa