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Antibodies

AMOT Antibody

Product Sizes
50 ul
£186.00
CSB-PA001677ESR2HU-50UL
100 ul
£334.00
CSB-PA001677ESR2HU-100UL
About this Product
SKU:
CSB-PA001677ESR2HU
Additional Names:
AMOT antibody; AMOT_HUMAN antibody; Angiomotin antibody; Angiomotin p130 isoform antibody; KIAA1071 antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
PBS
Clonality:
Polyclonal
Extra Details:
Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
Host:
Rabbit
Immunogen:
Recombinant Human Angiomotin protein (1-300AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot All lanes: Angiomotin antibody at 2ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 119, 73 kDa Observed band size: 119 kDa
Western Blot Positive WB detected in: A549 whole cell lysate All lanes: AMOT antibody at 1.21ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 119 kDa Observed band size: 130 kDa