Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

TSG101 Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-MA860355A0M-50UL
100 ul
£392.00
CSB-MA860355A0M-100UL
About this Product
SKU:
CSB-MA860355A0M
Additional Names:
ESCRT I complex subunit TSG101 antibody; ESCRT-I complex subunit TSG101 antibody; TS101_HUMAN antibody; TSG 10 antibody; TSG 101 antibody; TSG10 antibody; Tsg101 antibody; Tumor susceptibility 101 antibody; Tumor susceptibility gene 10 antibody; Tumor susceptibility gene 101 antibody; Tumor susceptibility gene 101 protein antibody; Tumor susceptibility protein antibody; Tumor susceptibility protein isoform 3 antibody; VPS 23 antibody; VPS23 antibody
Application:
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
6F4D11
Clonality:
Monoclonal
Host:
Mouse
Immunogen:
Recombinant Human TSG101 protein (1-145AA)
Isotype:
IgG1
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Monoclonal Antibody
Overlay Peak curve showing U251 cells stained with CSB-MA860355A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of U251 cells with CSB-MA860355A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: TSG101 antibody at 1:500 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 44, 32 KDa Observed band size: 44 KDa Exposure time : 5min