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Antibodies

MAGEA4 Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-MA013330A0M-50UL
100 ul
£392.00
CSB-MA013330A0M-100UL
About this Product
SKU:
CSB-MA013330A0M
Additional Names:
Cancer/testis antigen 1.4 antibody; CT1.4 antibody; MAGA4_HUMAN antibody; MAGE 4 antibody; MAGE 4 antigen antibody; MAGE 41 antibody; MAGE 41 antigen antibody; MAGE 4A antibody; MAGE 4B antibody; MAGE X2 antibody; MAGE X2 antigen antibody; MAGE-4 antigen antibody; MAGE-41 antigen antibody; MAGE-X2 antigen antibody; MAGE4 antibody; MAGE41 antibody; MAGE4A antibody; MAGE4B antibody; MAGEA 4 antibody; MAGEA4 antibody; Melanoma antigen family A 4 antibody; Melanoma associated antigen 4 antibody; Melanoma-associated antigen 4 antibody; MGC21336 antibody
Application:
ELISA, Flow Cytometry, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
1G10H5
Clonality:
Monoclonal
Host:
Mouse
Immunogen:
Recombinant Human Melanoma-associated antigen 4 protein (1-317AA)
Isotype:
IgG1
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Monoclonal Antibody
Overlay Peak curve showing A375 cells stained with CSB-MA013331A0m (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
IHC image of CSB-MA013330A0m diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-MA013330A0m diluted at 1:200 and staining in paraffin-embedded human bladder cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-MA013330A0m diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-MA013330A0m diluted at 1:200 and staining in paraffin-embedded human gastric cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.