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Antibodies

FLOT1 Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-MA008727A0M-50UL
100 ul
£392.00
CSB-MA008727A0M-100UL
About this Product
SKU:
CSB-MA008727A0M
Additional Names:
FLOT 1 antibody; FLOT1 antibody; FLOT1_HUMAN antibody; Flotillin-1 antibody; Flotillin1 antibody; Integral membrane component of caveolae antibody; Reggie 2 antibody
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
translate.label.attr.clone:
4C8B6
Clonality:
Monoclonal
Host:
Mouse
Immunogen:
Recombinant FLOT1 protein (149-427AA)
Isotype:
IgG2a
Physical State:
Liquid
Purification:
Protein A Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Monoclonal Antibody
Overlay Peak curve showing Hela cells stained with CSB-MA008727A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of Hela cells with CSB-MA008727A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
IHC image of CSB-MA008727A0m diluted at 1:100 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-MA008727A0m diluted at 1:100 and staining in paraffin-embedded human cervical cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, 3T3 whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue All lanes: FLOT1 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 48, 43 kDa Observed band size: 48 KDa Exposure time : 5min