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Antibodies

CYC1 Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-MA006327A0M-50UL
100 ul
£392.00
CSB-MA006327A0M-100UL
About this Product
SKU:
CSB-MA006327A0M
Additional Names:
Cytochrome c1, heme protein, mitochondrial (EC:7.1.1.8); Complex III subunit 4; Complex III subunit IV; Cytochrome b-c1 complex subunit 4; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; Cytochrome c-1
Application:
ELISA, Flow Cytometry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
27E12D11
Clonality:
Monoclonal
Host:
Mouse
Immunogen:
Recombinant Human Cytochrome c1 protein (85-325AA)
Isotype:
IgG1
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Monoclonal Antibody
Overlay Peak curve showing HepG2 cells stained with CSB-MA006327A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Western Blot Positive WB detected in: CYC1 antibody at 1:1000 Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: U251 whole cell lysate Lane 6: A549 whole cell lysate Lane 7: NIH/3T3 whole cell lysate Lane 8: PC-3 whole cell lysate Secondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 35 KDa Observed band size: 35 KDa Exposure time: 5min