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Antibodies

ABCB1 Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-MA001046A0M-50UL
100 ul
£392.00
CSB-MA001046A0M-100UL
About this Product
SKU:
CSB-MA001046A0M
Additional Names:
ABC20 antibody; ABCB1 antibody; ATP binding cassette, sub family B (MDR/TAP), member 1 antibody; ATP-binding cassette sub-family B member 1 antibody; CD243 antibody; CLCS antibody; Colchicin sensitivity antibody; Doxorubicin resistance antibody; GP170 antibody; MDR1 antibody; MDR1_HUMAN antibody; Multidrug resistance 1 antibody; Multidrug resistance protein 1 antibody; P glycoprotein 1 antibody; P gp antibody; P-glycoprotein 1 antibody; PGY1 antibody
Application:
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
translate.label.attr.clone:
5C12D3
Clonality:
Monoclonal
Host:
Mouse
Immunogen:
Recombinant Human Calnexin protein (350-710AA)
Isotype:
IgG2b
Physical State:
Liquid
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Monoclonal Antibody
Overlay Peak curve showing Hela cells stained with CSB-MA001046A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of HepG2 cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of Hela cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Western Blot Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate All lanes: ABCB1 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 141 kDa Observed band size: 141-250 KDa Exposure time : 5min