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Antibodies

Avi-Tag Monoclonal Antibody

Product Sizes
50 ul
£193.00
CSB-MA000101M0M-50UL
100 ul
£224.00
CSB-MA000101M0M-100UL
About this Product
SKU:
CSB-MA000101M0M
Application:
ELISA, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
9G3B5
Clonality:
Monoclonal
Host:
Mouse
Immunogen:
Synthetic Peptide
Isotype:
IgG2a
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Monoclonal Antibody
Immunoprecipitating Avi tag in 293F whole cell lysate transfected with avi tag Lane 1: Mouse control IgG instead of CSB-MA000101M0m in 293F whole cell lysate transfected with avi tag. Lane 2: CSB-MA000101M0m (1ug) + 293F whole cell lysate transfected with avi tag (500ug) Lane 3: 293F whole cell lysate transfected with avi tag(40ug) For western blotting, the blot was detected with CSB-MA000101M0m at 1:1000, and a HRP-conjugated Goat anti Mouse antibody was used as the secondary antibody at 1:50000
Western Blot Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli and Mammalian cell All lanes: Avi tag antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 40, 35, 30, 70, 40, 33, 35, 40 KDa Observed band size: 40, 35, 30, 70, 40, 33, 35, 4 KDa Exposure time : 1min
Western Blot Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli All lanes: Avi tag antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 81.2, 67, 66.9, 66.9 KDa Observed band size: 82, 67, 67, 67KDa Exposure time : 5min
Western Blot Positive WB detected in: Recombinant protein from Mammalian cell with Avi tag from E.coli at 100ng, 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng All lanes: Avi tag antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 40 KDa Observed band size: 40 KDa Exposure time : 1min
Western Blot Positive WB detected in: 50ng Recombinant protein with Avi tag from Mammalian cell Avi tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000, 1:12800000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 40 KDa Observed band size: 40 KDa Exposure time : 5min