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Antibodies

Myc tag Monoclonal antibody

Product Sizes
50 ug
£193.00
CSB-MA000041M0M-50UG
100 ug
£224.00
CSB-MA000041M0M-100UG
About this Product
SKU:
CSB-MA000041M0M
Additional Names:
bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
Application:
ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
5F92F9
Clonality:
Monoclonal
Extra Details:
Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein. It can help to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. Myc Tag also can be used to isolate protein complexes with multiple subunits. The Myc Tag Antibody is produced by the conjugation of a synthetic Myc tag peptide to KLH. Myc Tag Antibody can be used in various immunoassays, such as ELISA, Western blotting, immunoprecipitation, immunofluorescence, and more.
Host:
Mouse
Immunogen:
EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
Isotype:
IgG1
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Monoclonal Antibody
Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunoprecipitating MYC-tag with transfected HEK293 Lane 1: Mouse control IgG (1ug) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-MA000041M0m (6ug) + transfected HEK293 whole cell lysate (1mg) Lane 3: Transfected HEK293 whole cell lysate (10ug)
WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6ug/ml Lane 1: Recombinant Myc-tagged fusion protein at 50ng Lane 2: Recombinant Myc-tagged fusion protein at 25ng Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 50 kd Observed band size: 50 kd