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Antibodies

His Monoclonal Antibody

Product Sizes
50 ul
£193.00
CSB-MA000011M1M-50UL
100 ul
£224.00
CSB-MA000011M1M-100UL
About this Product
SKU:
CSB-MA000011M1M
Additional Names:
6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag
Application:
ELISA, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
2F4B5
Clonality:
Monoclonal
Host:
Mouse
Immunogen:
The immunogen is a mixture of various His-tagged proteins
Isotype:
IgG1
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Monoclonal Antibody
Immunoprecipitating His in 293 whole cell lysate transfected with His Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His. Lane 2: 2F4B5 antibody(2ug) + 293 whole cell lysate transfected with His (500ug) Lane 3: 293 whole cell lysate transfected with His (5ug) For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
Western Blot Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 43.4 KDa Observed band size: 43.4 KDa Exposure time : 5min
Western Blot Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size:62.5 KDa Observed band size: 66 KDa Exposure time : 5min
Western Blot Positive WB detected in:200ng of four Recombinant protein with his tag All lanes: His antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 43.4, 62.5,56,80.6 KDa Observed band size:43.4, 66, 57 ,90 KDa Exposure time : 5min