Anti-Collagen 4 Cocktail monoclonal antibody (Clones H53, B51, H25)

Product Sizes

1 ml

SGE-CFT45325-1ML

About this Product

SKU:

SGE-CFT45325

Application:

Immunofluorescence

translate.label.attr.clone:

Clones h53

Clonality:

Monoclonal

Extra Details:

Check out our Human Collagen IVA Alpha Rat Monoclonal Antibodies Dashboard for more information about this product and additional epitope-defined monoclonal antibodies useful for research on hereditary diseases related to collagen IV. Alport syndrome is an inherited disease characterized by the pathological absence or reduction of the collagen A Alpha5(IV) chain in glomerular basement membrane (GBM), tubular basement membrane (TBM) and Bowman's capsular basement membrane. Anti-Collagen 4 Cocktail mAb (cat no. SGE-CFT45325) is a cocktail of three different rat mAbs for easy and rapid staining of human renal and skin biopsy sections to distinguish Alport syndrome from normal tissue. It comprises two different FITC-conjugated mAbs (clones H53 and B51) that reveal the Alport-affected A Alpha5(IV) chain in glomerular, tubular and Bowman's capsular BMs and an internal positive control Texas Red-conjugated mAb (clone H25) that targets A Alpha2(IV) to reveal endothelial basement membrane structure Product specifications References

Kagawa M et al. Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport syndrome. Nephrol. Dial. Transplant. 12: 1238-1241 (1997) (PMID: 9198058) Borza DB et al. The NCI domain of collagen IV encodes a novel network composed of the A Alpha1, A Alpha2, A Alpha5, and A Alpha6 chains in smooth muscle basement membranes. J. Biol. Chem. 276: 28532-28540 (2001) (PMID: 11375996) Sado et al. Establishment by the rat lymph node method of epitope-defined monoclonal antibodies recognizing the six different A Alpha chains of human type IV collagen. Histochem. Cell Biol. 104: 267-275 (1995) (PMID: 8548560) Yoshioka K et al. Type IV collagen A Alpha5 chain: Normal distribution and abnormalities in X-linked Alport syndrome revealed by monoclonal antibody. Am. J. Pathol. 144: 986-996 (1994) (PMID: 8178947) Ninomiya Y et al. Differential expression of two basement membrane collagen genes, COL4A6 and COL4A5, demonstrated by immunofluorescence staining using peptide-specific monoclonal antibodies. J. Cell Biol. 130: 1219-1229 (1995) (PMID: 7657706) Naito I et al. Relationship between COL4A5 gene mutation and distribution of type IV collagen in male X-linked Alport syndrome. Kidney Int. 50: 304-311 (1996) (PMID: 8807602)Citations

Bu L et al. Somatic Mosaicism in a Male Patient With X-linked Alport Syndrome. Kidney Int Rep. 14(4): 1031-1035 (2019) (PMID: 31312776) Samar M et al. Negative Staining for COL4A5 Correlates With Worse Prognosis and More Severe Ultrastructural Alterations in Males With Alport Syndrome. Kidney Int Rep. 2(1): 44-52 (2017) (PMID: 29142939) Malone AF et al. Functional assessment of a novel COL4A5 splice region variant and immunostaining of plucked hair follicles as an alternative method of diagnosis in X-linked Alport syndrome. Pediatr Nephrol. 32(6): 997-1003 (2017) (PMID: 28013382) Nozu K et al. X-linked Alport syndrome caused by splicing mutations in COL4A5. Am Soc Nephrol. 9(11): 1958-64 (2014) (PMID: 25183659) Matsubara S et al. Pregnancy complicated with Alport syndrome: a good obstetric outcome and failure to diagnose an infant born to a mother with Alport syndrome by umbilical cord immunofluorescence staining. Obstet Gynaecol Res. 35(6): 1109-14 (2009) (PMID: 20144175) Patey-Mariaud de Serre N et al. Collagen alpha5 and alpha2(IV) chain coexpression: analysis of skin biopsies of Alport patients. Kidney Int. 72(4): 512-6 (2007) (PMID: 17554254) Kharrat M et al. Autosomal dominant Alport's syndrome: study of a large Tunisian family. Kidney Dis Transpl. 17(3): 320-5 (2006) (PMID: 16970251), SGE

Host:

Rat

Immunogen:

Collagen 4 Cocktail

Reactivities:

Human

Shipping Conditions:

Blue Ice

Storage Conditions:

2-8[o]C

Supplier: