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Antibodies

Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) monoclonal antibody (Clone LC3-1703)

Product Sizes
50 ug
£714.00
CAC-CTB-LC3-2-IC-50UG
About this Product
SKU:
CAC-CTB-LC3-2-IC
Application:
Immunocytochemistry, Immunoelectron Microscopy, Immunofluorescence
translate.label.attr.clone:
Clone lc3-1703
Clonality:
Monoclonal
Extra Details:
LC3 is a mammalian autophagosome marker. It is immediately cleaved to form LC3-I by Atg4, and when autophagy is induced, phosphatidylethanolamine is covalently linked to LC3-I C-terminal glycine to form LC3-II. LC3-II is membrane-bound and thought to be present in autophagosomal membranes. Since autophagosomes are degraded by fusion with lysosomes, LC3-II itself is also degraded by autophagy. Therefore, it is generally accepted that the amount of LC3-II correlates well with the amount of autophagosomes. This antibody shows good results in ICC and Immunoelectron microscopy experiments. Source: Professor Noboru Mizushima, Department of Cell Physiology, Tokyo Medical and Dental University References: 1) Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y. and Yoshimori, T. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing EMBO J. 19, 5720-5728. (2000) 2) Mizushima, N., Yoshimori, T. How to interpret LC3 immunoblotting Autophagy 3:542-545 (2007) 3) Mizushima, N., Yoshimori, T. and Levine, B. Methods in mammalian autophagy research. Cell 140; 313-326 (2010), CAC
Host:
Mouse
Immunogen:
Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3)
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Storage Conditions:
-20[o]C
Supplier:
Cosmo Bio Ltd
Type:
Antibody: Monoclonal Antibody