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Antibodies

Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) monoclonal antibody (Clone LC3.No.6)

Product Sizes
50 ug
£595.00
CAC-CTB-LC3-1-50-50UG
About this Product
SKU:
CAC-CTB-LC3-1-50
Application:
Western Blot
translate.label.attr.clone:
Clone lc3.no.6
Clonality:
Monoclonal
Extra Details:
LC3, a mammalian homolog of yeast Atg8, is used as a marker for autophagic vesicles (autophagosomes) formed in the process of autophagy. Immediately after synthesis, LC3 is cleaved by Atg4 to form LC3-I. When autophagy is induced phosphatidylethanolamine is covalently linked to the C-terminal glycine of LC3-I to form membrane-bound LC3-II. Most LC3-II is thought to be present in autophagosomal membranes. Since autophagosomes are degraded by fusion with lysosomes, LC3-II itself is also degraded by autophagy. Therefore, it is generally accepted that the amount of LC3-II correlates well with the amount of autophagosomes. This antibody reacts with both LC3 isoforms (LC3-I and LC3-II) and shows good results in Western blot experiments. References: 1) Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y. and Yoshimori, T. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing EMBO J. 19, 5720-5728. (2000). 2) Mizushima, N., Yoshimori, T. How to interpret LC3 immuno, CAC
Host:
Mouse
Immunogen:
Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3)
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Storage Conditions:
-20[o]C
Supplier:
Cosmo Bio Ltd
Type:
Antibody: Monoclonal Antibody