S-Adenosylmethionine (SAM) ELISA Kit
Product Sizes
96 assays
MET-5152-96ASSAYS
About this Product
- SKU:
- MET-5152
- Extra Details:
- S-adenosylmethionine (SAM) is a substrate for methyl transfers to various biomolecules that yields S-adenosylhomocysteine (SAH). SAH is then hydrolyzed to homocysteine and adenosine; which is converted to methionine; and ultimately back to SAM. SAH can act as a methylation inhibitor; therefore the SAM/SAH ratio; or methylation potential; is important for maintaining proper methylation in the cell. Our S-adenosylmethionine (SAM) ELISA is designed for detection and quantitation of SAM in plasma; serum; lysates; or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAM antibody are added to a plate coated with a SAM conjugate. The sample and conjugate compete for antibody binding; which generates a reverse curve. Samples with high SAM levels will bind the majority of the antibody; which gets washed away and results in a low OD; while samples with low SAM levels will leave more antibody available to bind to the conjugate; producing a high signal.
- Format:
- Colorimetric
- Physical State:
- Cell Lysate, Other Biological Fluid, Plasma, Serum, Tissue Lysate
- Research Area:
- Metabolism Research
- Sensitivity:
- 0.625 nM
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- Upon receipt; store SAM Conjugate and SAM Standard at -80ºC. Store the rest of the kit at 4ºC.
- Supplier:
- Cell BioLabs, Inc.
- Type:
- Assay Kits: ELISAs
